remove_batch_effects() removes batch effects from the data using the limma package.
It converts the dataframe into matrix and transposes it to get it ready for limma.
It removes the batch effects and then converts the data back to tibble.
Arguments
- wide_data
A tibble containing the data to be normalized. The data should be in wide format.
- metadata
A tibble containing the metadata information.
- sample_id
The column containing the sample ID information.
- batch
The metadata column containing the batch information.
- batch2
The metadata column containing the second batch information. Default is NULL.